c-Myc T58A mutant significantly induced tumor formation in nude mice.

<p>Ba/F3 cells were infected with empty virus (-) and retrovirus encoding wild-type c-Myc (c-Myc) or two c-Myc mutants (T58A, In373). Transduced Ba/F3 cells and VF/EpoR cells were s.c. injected into nude mice (1×10⁷ cells/mice). (A) Nude mice were photographed 18 days post-inoculation. Arrows indicate tumors in nude mice. (B, C) Eighteen days post-inoculation, three mice were sacrificed. The morphology of the liver and spleen were photographed. The weights of the spleen and liver were measured and shown in the graphs. * indicates significant difference p<0.01 (n = 3). (D) Sixty days after inoculation, sections of the spleen and liver were stained with hematoxylin and eosin (magnification: ×100). (E) Mouse survival was monitored daily for 100 days post-inoculation (n = 10).