The effect of EZH2 down-regulation on anchorage-independent growth and invasion of NSCLC cells.

<p>A549 or 1299 were transfected with EZH2-targeting siRNA or scrambled siRNA control for 6 hours. (A) For anchorage-independent colony formation assay, the transfected cells were seeded in 0.35% agarose in a 6-well plate at 2×10⁴/well. The colonies formed were counted after 3-weeks incubated with regular change of medium. (B) For invasion and migration assay, 1×10⁵ transfected cells in 1% serum were seeded on top of a BD BioCoat Matrigel invasion chamber with 10% FBS in the lower chamber. After 24 hours, the cells were fixed with 4% formaldehyde with 0.5% crystal violet. Cells invaded to the lower chamber were counted under low magnification microscope. The experiments were run in duplicate and repeated three time. The average number and standard derivation of each treatment were graphed here.