EZH2 knockdown causes a significant reduction in SCLC cell viability.

<p>(<b>A, E</b>) To confirm knockdown of EZH2, RNA was extracted from the transfected lines and qPCR was performed. Expression in knockdown lines (E1) is shown relative to PLKO controls. (<b>B,F</b>) Confirmation of protein levels of EZH2 in the knockdown lines was performed using standard Western blotting methods. Both qPCR and Western blotting verified EZH2 levels were reduced by over 50% in HTB-175 and H524 cells upon shRNA-mediated knockdown. (<b>C,D,G,H</b>) To assess the viability of <i>EZH2</i> knockdown relative to controls cells we performed MTT assays. Knockdown of EZH2 caused a significant and reproducible decrease in cell viability in both SCLC cell lines (Student's t-test, p<0.05).