Immnoblot assessment of EZH2 down-regulation on the expression of cell cycle regulators and apoptotic markers.

<p>H1299 (A) or A549 (B) cells were transfected with EZH2-targeting siRNA or scrambled siRNA control as indicated. 72 hours post transfection, the cells were harvested and total protein was extracted. Twenty micrograms of protein per well were separated on SDS-PAGE and transferred to nitrocellulose membrane. The blot was probed with the indicated antibody and visualized by HRP-conjugated secondary antibody and ECL. Images on film were scanned using Canon film scanner. The expression level were quantified by digitized pixel density count using Adobe Photoshop and expressed as fold changes beneath the images.