Functional analyses of lung DCs derived from <i>Bordetella</i> infections.

<p>(<b>A</b>) <b>Transwell CCR7 dependent migration of lung DCs</b>. 5×10⁵ isolated lung DCs from <i>Bordetella</i> infections were placed in the transwell upper chamber and allowed to migrate through a 5 µm polycarbonate membrane in the presence or absence of chemokines. Migrated cells were harvested, stained for CD11c & MHC-II, and enumerated by flow cytometry. Chemotaxis index data are calculated as indicated in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0052903#s2" target="_blank">Materials and Methods</a>. Depicted are combined data of three experiments. In each experiment, five mice were used per treatment group. *: p<0.05 as analyzed by a two-tailed student's <i>t</i>-test. (<b>B</b>) <b>Enumeration of CD11c⁺ cells in the mediastinal lymph nodes.</b> The mediastinal lymph nodes were harvested, stained with CD11c and CD45, and enumerated with Trubeads by flow cytometry. The absolute number of CD11c⁺ cells from each treatment was normalized to that from <i>B. pertussis</i> (1.0 by definition) as detailed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0052903#s2" target="_blank">Materials and Methods</a>. The bar chart represents compiled data with error bars showing the SEM of three independent experiments. In each experiment, three mice were used per treatment group. *: p<0.05, and no asterisk indicates p<0.05 as analyzed by the one sample two-tailed student's <i>t</i>-test.