Characterization of lung CD11c⁺ cells during infection with <i>B. pertussis</i> and <i>B. parapertussis</i>.

<p>(<b>A</b>) Enumeration of lung CD11c⁺ cells during the early phase of infection with <i>Bordetella sp</i>. Mononuclear cells were isolated from lungs and enriched for DCs (CD11c⁺) as detailed. After column enrichment, the total number of CD11c⁺ cells per mouse was estimated using a hemocytometer. (<b>B</b>) CD11c⁺ enriched lung mononuclear cells were gated on singlets and then on CD11c⁺ cells (DCs). The frequency of CD11c⁺ cells expressing maturation markers was assessed at 5 days p.i. (<b>C</b>) and at 25 days p.i. (<b>D</b>), and normalized to the respective cells in uninfected control mice (1.0 by definition). In addition, the frequency of lung CD11c⁺ cells expressing CCR7 and CXCR4 at 5 days p.i. was measured (<b>E</b>). The bar charts (<b>C, D, E</b>) represent compiled data with error bars showing the SEM of four independent experiments. In each experiment, six mice were used per treatment group. *: p<0.05, and no asterisk indicates p<0.05 as analyzed by a two-tailed student's <i>t</i>-test.