α4β7 and α4β1 imprinting on allogeneic Thy1.1⁺/CD4⁺/CD38⁺⁺ cells.

<p>Lung DCs from 5 days p.i. with one of four infection types—<i>B. pertussis</i>, <i>B. parapertussis</i>, <i>BpΔPTX</i>, or uninfected controls—were co-cultured for 4 days with naïve allogeneic Thy1.1⁺/CD4⁺ splenocytes at a 1∶5 ratio. (<b>A</b>) Lung DCs from <i>Bordetella</i> infections were used to stimulate purified naïve Thy1.1⁺/CD4⁺ splenocytes labeled with CFSE. Histograms depict CFSE dilution profiles. Approximately four cell cycles of division are observed in each system as estimated by FlowJo proliferation analysis. Low mRMS values obtained in every allogeneic system confirmed the accuracy of the analysis. (<b>B</b>) Co-cultured cells were gated on lymphocytes, followed by gating on singlets, and then on Thy1.1⁺/CD4⁺/CD38⁺⁺ cells. (<b>C</b>) Proportion of Thy1.1⁺/CD4⁺/CD38⁺⁺ cells expressing α4β7 or α4β1 following co-culture with lung DCs derived from <i>Bordetella</i> infections was measured and normalized to the respective cells from the uninfected control co-culture (1.0 by definition). The bar charts represent compiled data with error bars showing the SEM of three independent experiments. In each experiment, five mice were used per treatment group. *: p≤0.05, no asterisk: p<0.05.