Effects of DOX on cellular apoptosis in MCF-7 and MDA-MB-231 cells.

<p>The apoptosis rate was detected by flow cytometry using the Annexin V-fluorescein isothiocyanate/propidium iodide (PI) kit, and the Annexin V-positive, PI-negative cells were scored as early apoptotic. The apoptosis rates estimated in the present study only included the early apoptotic cells which were marked as LR. A shows the alternation of apoptosis rate in MCF-7 cells and B displays that in MDA-MB-231 cells. A21, cells treated with 0.2 µmol/L DOX for 24 h; An, cells transfected with Sp1 ODNs for 24 h; AnA, cells treated with 0.2 µmol/L DOX for 24 h after their transfection with Sp1 ODNs; Mu, cells transfected with mock Sp1 ODNs for 24 h; MuA, cells treated with 0.2 µmol/L DOX for 24 h after they were transfected with mock Sp1 ODNs.