Flow cytometry characterization of bone marrow derived macrophages (BMDM) <i>vs</i>. alveolar macrophages (ALVM).

<p>Cells were isolated from either bone marrow obtained from Fischer 344 rat femurs (panel A, C, E) or following bronchioalveolar lavage (panel B, D, F) and cultured as described (n = 3 rats for each determination). Upon reaching optimal differentiation, macrophages were stained with Alexa Fluor 647 (AF 647) conjugated mouse anti-rat CD11b (OX-42), fluorescein isothiocyanate (FITC) conjugated mouse anti-rat CD11b (WT.5), or phycoerythrin (PE) conjugated mouse anti-rat CD172 [right column] or respective isotype control [left column] and analyzed by flow cytometry. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0047639#s3" target="_blank">Results</a> are representative of two independent experiments.