Effect of Hbp knockdown on survival of <i>B. henselae</i> in endothelial cells.

<p>Survival of <i>B. henselae</i> pNS2Trc and <i>B. henselae</i> pNS2Trc<i>::hbps_AS</i> in endothelial cell. After gentamicin killing, infected cell were grown for 24 h or 48 h in modified DMEM medium. Lysates were incubated in Schneider’s medium overnight. Then lysates were collected after centrifugation and plated on CBA blood plates to determine the number of intracellular bacteria. The survival rates after 24 h growth (A) and 48 h growth (B) were calculated using the equation provided in Materials and methods. (*P<0.05, **P<0.01, ***P<0.005 compared to <i>B. henselae</i> pNS2Trc).