<p>J774 cells and neutrophils were infected with 100 or 10 MOI of <i>A. baumannii</i>, respectively, and extracellular bacteria were killed by gentamycin as detailed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0040019#pone-0040019-g004" target="_blank">Figure 4</a>. The cells were lysed at indicated times (hours) after infection to determine viable bacterial counts by quantitative bacteriology. Data are expressed as the percentage of the ingested bacteria that were killed by J774 cells or neutrophils at indicated time and presented as mean ± SD (n = 3 for J774 macrophages and n = 9 for neutrophils). The neutrophil data are compiled from three independent experiments.</p
<p>A549 cells were infected with <i>A</i>. <i>baumannii</i> wild-type or mutant cells for 5 hours. T...
<p>Bone marrow (A-B and E-F) or peritoneally-recruited (C-D and G-J) neutrophils were isolated from ...
<p>Vaccinated and control mice were infected with 2.25×10<sup>6</sup> cfu (340.9× LD<sub>50</sub>) o...
<p>J774 cells were pretreated for 1 h with various concentrations of the indicated biochemical inhib...
<p>J774 (5×10<sup>5</sup> cells) cells were infected with 100 MOI of <i>A. baumannii</i> in 500 μl D...
<p>J774 cells were infected with <i>A. baumannii</i> (MOI = 100) for 2 h. The cells were then rinsed...
Acinetobacter baumannii is one of the most antibiotic-resistant pathogens. Defining mechanisms drivi...
Background. Acinetobacter baumannii is one of the most antibiotic-resistant pathogens. Defining mech...
<p>Survival of mice after intratracheal infection with <i>A. baumannii</i> clone I strain RUH875, cl...
<p>J774 cells were treated with 2 mM iNOS inhibitors (L-NAME or L-NMMA) or controls (D-NAME or media...
<p>A) Determination of the LD<sub>50</sub> of <i>A. baumannii</i> ATCC 17978. Groups of 5 mice were ...
<p>Groups of C57BL/6 mice were i.n treated with either clodronate-liposomes to deplete alveolar macr...
<p>The monocyte-derived macrophage cell line THP-1 or freshly isolated granulocytes were infected wi...
<p>Survival of bacteria was monitored by enumerating cfu's following 4-h incubation to 10<sup>3</sup...
Background: The dramatic increase in antibiotic resistance and the recent manifestation in war traum...
<p>A549 cells were infected with <i>A</i>. <i>baumannii</i> wild-type or mutant cells for 5 hours. T...
<p>Bone marrow (A-B and E-F) or peritoneally-recruited (C-D and G-J) neutrophils were isolated from ...
<p>Vaccinated and control mice were infected with 2.25×10<sup>6</sup> cfu (340.9× LD<sub>50</sub>) o...
<p>J774 cells were pretreated for 1 h with various concentrations of the indicated biochemical inhib...
<p>J774 (5×10<sup>5</sup> cells) cells were infected with 100 MOI of <i>A. baumannii</i> in 500 μl D...
<p>J774 cells were infected with <i>A. baumannii</i> (MOI = 100) for 2 h. The cells were then rinsed...
Acinetobacter baumannii is one of the most antibiotic-resistant pathogens. Defining mechanisms drivi...
Background. Acinetobacter baumannii is one of the most antibiotic-resistant pathogens. Defining mech...
<p>Survival of mice after intratracheal infection with <i>A. baumannii</i> clone I strain RUH875, cl...
<p>J774 cells were treated with 2 mM iNOS inhibitors (L-NAME or L-NMMA) or controls (D-NAME or media...
<p>A) Determination of the LD<sub>50</sub> of <i>A. baumannii</i> ATCC 17978. Groups of 5 mice were ...
<p>Groups of C57BL/6 mice were i.n treated with either clodronate-liposomes to deplete alveolar macr...
<p>The monocyte-derived macrophage cell line THP-1 or freshly isolated granulocytes were infected wi...
<p>Survival of bacteria was monitored by enumerating cfu's following 4-h incubation to 10<sup>3</sup...
Background: The dramatic increase in antibiotic resistance and the recent manifestation in war traum...
<p>A549 cells were infected with <i>A</i>. <i>baumannii</i> wild-type or mutant cells for 5 hours. T...
<p>Bone marrow (A-B and E-F) or peritoneally-recruited (C-D and G-J) neutrophils were isolated from ...
<p>Vaccinated and control mice were infected with 2.25×10<sup>6</sup> cfu (340.9× LD<sub>50</sub>) o...