Effect of <i>hbps</i> knockdown on growth of <i>B. henselae</i> on blood plates.

<p>For the growth test on CBA plates, strains <i>B. henselae</i> (pNS2Trc), <i>B. henselae</i> (pNS2Trc<i>::hbpA _AS</i>), <i>B. henselae</i> (pNS2Trc<i>::hbpB _AS</i>), <i>B. henselae</i> (pNS2Trc<i>::hbpC _AS</i>) and <i>B. henselae</i> (pNS2Trc<i>::hbpD_AS</i>) were collected after 5 days of growth on CBA plates and suspended in PBS buffer to obtain about 10³ CFU ml⁻¹. Two-hundred microliters of cell suspension were plated on the CBA plate. Colony sizes were measured after 6 and 10 days of growth at 35°C in the presence of 5% CO₂. Data are the mean diameter (mm) ± SD of 10 colonies from one representative experiment. Standard deviations were calculated using Statview software. All experiments were repeated three times. NM: not measurable.