Affinity chromatography of milk pIgGs on two different resins.

<p>Various IgG fractions were separated using DNA-cellulose (A) and ATP-Sepharose (B): (–), absorbance at 280 nm; symbols correspond to the relative catalytic activities (RA) in the hydrolysis of DNA (▵), ATP (○), oligosaccharides (×); phosphorylation of lipids (▪) and polysaccharides (⋄) tightly bound with IgGs. Depending on the RA and reaction analyzed, the reaction mixtures were incubated for 0.5–2 h and then the RAs were normalized to the standard conditions and the RA of the fraction with the highest activity was taken for 100%. The average error in the initial rate determination from two experiments in each case did not exceed 7–10%.