Affinity chromatography of non-modified and FITC-modified pIgG_mix on DNA-cellulose.

<p>(–) and (–), absorbance of IgGs at 280 nm before and after modification of IgGs with FITC, respectively; the bars correspond to the relative fluorescence of FITC-IgG_mix fractions (A). Analysis of a relative efficiency of specific-molecule exchange under different conditions between non-modified IgG_mix and FITC-IgG_mix having different affinity for DNA-cellulose (B–D). Before chromatography, the IgG_mix eluted from DNA-cellulose by 0.15 M NaCl (0.15 M-IgG_mix) were incubated with FITC-IgG_mix eluted by 1.5 M NaCl (1.5 M-FITC-IgG_mix) in the presence of TBS and GSH (B); 0.5 M-IgG_mix and 1.5 M-FITC-IgG_mix (C) or 0.5 M-FITC-IgG_mix +1.5 M-IgG_mix (D) were incubated in the presence of TBS containing GSH and human milk plasma.