SDS-PAGE analysis of FITC-modified pIgG_mix formation.

<p>The analysis was performed after non-labeled pIgG_mix incubation with preparations of separated FITC-modified heavy (A, B) and light chains (C, D) of Abs. The relative fluorescence of proteins (A and C) and their position on the gel (B and D; Coomassie staining) were analyzed. Before electrophoresis, FITC-heavy-chains were incubated alone (lanes 1, A and B), in the presence of the IgG_mix and GSH (lanes 2, A and B) or in the presence of the IgG_mix, GSH and human milk plasma (lanes 3, A and B). Lane 4 (B) corresponds to human milk plasma incubated alone. FITC-L-chains were incubated alone (lanes 1, C and D), in the presence of the IgG_mix and GSH (lanes 2, C and D) or in the presence of the IgG_mix, GSH, and human milk plasma (lane 3 C and D). The arrows (lane 4, D) indicate the positions of molecular mass markers. For details, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0042942#s4" target="_blank">Materials and methods</a>.