Competition assays.

<p>(A) In inhouse MCV IgM EIA, biotinylated MCV VLPs (10 ng/100 µL) were mixed with increasing concentrations of either unbiotinylated MCV VLPs or soluble biotin and added onto the µ-capture plates (100 µL/well). (B) The upper figures show two serum samples positive for biotin IgM; the lower figures show two serum samples positive for MCV IgM. <b>(C and D)</b> In Bio-EIA, four samples positive for biotin IgM were coincubated with avidin and applied to the assay. Five-pointed star: HRP conjugates.