In a model ELISA system alkaline phosphatase (AP) absorbed onto microtitre wells was employed as the target antigen. The antigen was then reacted with a monoclonal antibody to AP either unlabelled or labelled with (a) FITC and (b) biotin. The bound anti-AP was then detected with horseradish peroxidase (HRP) conjugates of polyvalent anti-mouse IgG, and the FITC and biotin anti-AP conjugates with HRP conjugates of a monoclonal anti-FITC or streptavidin respectively. The FITC-anti-FITC system proved to be of similar sensitivity to the biotin-streptavidin system detecting 140 amol compared to 350 amol of antigen. Both these methods of antigen detection were superior to the anti-mouse IgG reagent (2100 amol). In contrast to biotinylated antibodi...
A novel screening method was established to select new monoclonal antibodies which react with unknow...
The purpose of this investigation was to create and optimise a capture assay for the detectionof ant...
<p>(A) hESCs were preincubated with biotinylated 297-D4 in the presence of isotype control antibody ...
Department of Biological SciencesSince enzyme immunoassays have been used for biological samples amo...
The aim of this study was to produce anti-TNP monoclonal antibodies (MAbs) that could be conjugated ...
The aim of this study was to produce anti-TNP monoclonal antibodies (MAbs) that could be conjugated ...
<p>Cells were incubated with 100 pmol of the respective soluble HA or the control FcATG. Soluble HAs...
(canine hyperimmune serum) as the primary antibody, followed by a detection system with a secondary...
<p>(a) Assaying the formation of Nedd8∼NAE thioester conjugate immobilized to the streptavidin plate...
Biotin-binding human immunoglobulin G (B-IgG) was detected by two different methods in the avidin-co...
Labeled avidin-biotin (LAB) enzyme-linked immunosorbent assay (ELISA) was applied to detect Japanese...
<p>The 1:500 dilution of biotinylated antibody was detected by streptavidin-HRP mixed with different...
<p>96-well ELISA plates were coated with purified GST-MART-1 and incubated with purified 1F9, 2A9 or...
<p>Complete schematics are displayed. ESAT-6 protein was coated onto the plate followed by anti-ESAT...
<p>(A) In inhouse MCV IgM EIA, biotinylated MCV VLPs (10 ng/100 µL) were mixed with increasing conce...
A novel screening method was established to select new monoclonal antibodies which react with unknow...
The purpose of this investigation was to create and optimise a capture assay for the detectionof ant...
<p>(A) hESCs were preincubated with biotinylated 297-D4 in the presence of isotype control antibody ...
Department of Biological SciencesSince enzyme immunoassays have been used for biological samples amo...
The aim of this study was to produce anti-TNP monoclonal antibodies (MAbs) that could be conjugated ...
The aim of this study was to produce anti-TNP monoclonal antibodies (MAbs) that could be conjugated ...
<p>Cells were incubated with 100 pmol of the respective soluble HA or the control FcATG. Soluble HAs...
(canine hyperimmune serum) as the primary antibody, followed by a detection system with a secondary...
<p>(a) Assaying the formation of Nedd8∼NAE thioester conjugate immobilized to the streptavidin plate...
Biotin-binding human immunoglobulin G (B-IgG) was detected by two different methods in the avidin-co...
Labeled avidin-biotin (LAB) enzyme-linked immunosorbent assay (ELISA) was applied to detect Japanese...
<p>The 1:500 dilution of biotinylated antibody was detected by streptavidin-HRP mixed with different...
<p>96-well ELISA plates were coated with purified GST-MART-1 and incubated with purified 1F9, 2A9 or...
<p>Complete schematics are displayed. ESAT-6 protein was coated onto the plate followed by anti-ESAT...
<p>(A) In inhouse MCV IgM EIA, biotinylated MCV VLPs (10 ng/100 µL) were mixed with increasing conce...
A novel screening method was established to select new monoclonal antibodies which react with unknow...
The purpose of this investigation was to create and optimise a capture assay for the detectionof ant...
<p>(A) hESCs were preincubated with biotinylated 297-D4 in the presence of isotype control antibody ...