PTHrP NLS and C terminus deficiency results in a reduction of neurosphere forming efficiency.

<p>(A) Representative micrographs of neurospheres developed from the neurosphere assay of WT and KI mice. (B) Neurosphere numbers per well developed from 3, 6 and 9 day cultures. (C) Neurosphere numbers per well at different sizes developed from 9 day cultures. *, <i>P</i><0.05; **, <i>P</i><0.01; ***, <i>P</i><0.001 in <i>Pthrp</i> KI mice relative to wild-type littermates. <b>##</b>, <i>P</i><0.01; <b>###</b>, <i>P</i><0.001 at the time point relative to the prior observed time point in WT mice. <b>▴</b>, <i>P</i><0.05 at the time point relative to the prior observed time point in <i>Pthrp</i> KI mice.