Functional validation of identified molecules <i>in vitro</i> neurosphere culture.

<p>(A) The number of neurospheres. In the presence of Ttr and CPE, fewer neurospheres formed, while Enpp2 and Sparcl1 treatment did not show significant differences (Ttr: p = 0.012, n = 4; CPE: p<0.0001, n = 3). (B) The size of neurospheres determined by the neurosphere diameter. In the presence of Ttr and CPE, smaller neurospheres were formed, while Enpp2 and Sparcl1 treatment did not show significant differences (Ttr: p = 0.0072, n = 4; CPE: p = 0.031, n = 3). (C) XTT cell proliferation assay. Ttr and CPE treatment resulted in less number of total cells in culture wells compared to the control, while Enpp2 and Sparcl1 treatment did not show significant differences (Ttr: p = 0.017, CPE: p = 0.020; n = 3). Each symbol represents the mean of triplicates from each independent experiment. Bars are the mean of 3 or 4 such independent experiments. Symbols with the same color in the graph are the data set of each independent experiment.