Reduced proliferation and neuronal differentiation in neurospheres isolated from adult <i>C/EBPβ^−/−</i> compared to <i>C/EBPβ^+/+</i> mice.

<p>(A) Representative Western blot showing the presence of the different C/EBPβ isoforms (LAP1, LAP2 and LIP) in NS cultures established from adult mice. (B) Immunocytochemistry analysis showing the BrdU incorporation in <i>C/EBPβ^−/−</i> and <i>C/EBPβ^+/+</i> NS cultures plated for 24 h in proliferation medium. Quantification analysis show a significantly reduction in the number of BrdU-positive cells in <i>C/EBPβ^−/−</i> cultures compared to wild type NS cultures. (C) <i>C/EBPβ^−/−</i> and <i>C/EBPβ^+/+</i> NS cultures were plated for 72 h in differentiation medium and MAP2 immunocytochemistry was performed as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0024842#s4" target="_blank">Material and Methods</a>. Quantification analysis shows that the percentage of MAP2 positive cells in <i>C/EBPβ^−/−</i> NS cultures was significantly reduced. Nuclei were counterstained with Hoechst (blue). Scale bars, 25 µm. Values represent the mean ± S.E. <i>**P</i>≤0.01.