Detection of endogenous XBP-1 in mouse oocytes and preimplantation embryos <i>in vivo</i>.

<p>A. A specific anti-XBP-1 antibody was used to detect localization of XBP-1 in mouse oocytes via immunostaining (green). Nuclei were stained with DAPI (blue); Scale bar, 20 µm. B. Confocal immunofluorescence images of mouse pre-implantation embryos. The XBP-1 protein was detected using a specific antibody (green). Negative control embryos were probed directly with the secondary antibody. Nuclei were stained with DAPI (blue); Scale bar, 20 μm.