Detection of the OT system in the E15 mouse embryo by immunocytochemistry.

<p>Somite staining with OT-GKR antibody (<b>A</b>) and selective antibodies for OT (<b>B</b>) and OTR (<b>C</b>). TUNEL reaction displaying apoptosis (<b>D</b>)<b>.</b> Control staining of somites with OT-GKR-specific antibody reabsorbed with OT-GKR (<b>E</b>) Control staining of whole embryo with OT-GKR-specific antibody reabsorbed with OT-GKR (<b>F</b>). Immunodetection of OT-GKR in the fetal mouse heart at day E15 (arrow) (<b>G</b>). Polyclonal rabbit antibody specifically recognizing OT-GKR peptide was applied to detect OT-GKR. Staining was revealed by the biotin-streptavidin method. Higher magnification of OT-GKR staining in the fetal heart (<b>H</b>). Immunofluorescence of OT-GKR (Texas Red) in cryostat sections section of cardiac tissue (stained green by troponin Alexa Fluor 488 antibody) by (<b>I</b>)<b>.</b> Immunodetection in the heart of OTR (<b>J</b>)<b>,</b> OT (<b>K</b>), AVP (<b>L</b>), and V1R (<b>M</b>)<b>.</b> Control staining with OT-GKR-specific antibody reabsorbed with OT-GKR (<b>N</b>).