Dose-dependent effects of stress inducers on localization of XBP-1.

<p>A. Two-cell stage embryos cultured with activator or inhibitor of ER stress were examined using a specific anti-XBP-1 antibody (green). Nuclei were stained with DAPI (blue). Scale bar, 20 μm. B. Active XBP-1 in two-cell embryos in the presence of activator or inhibitor of ER stress was analyzed using Western blotting. β-actin served as the control. Each experiment was repeated three times. (*) indicates a statistically significant difference from control (P<0.05).