ISO significantly increased I_Ca-T(3.1) and I_Ca-L.

<p><b>A</b>, Raw current recordings at −40 mV from the V_h of −90 mV before and after ISO. <b>B</b>, Time course of the change of the amplitude of I_Ca measured at the test potential of −40 mV from the V_h = −90 mV, which was almost completely consisting of I_Ca-T(3.1). <b>C-E</b>, Averaged I–V curves of total I_Ca (<b>C</b>), I_Ca-L (<b>D</b>), and I_Ca-T (<b>E</b>) before and after ISO application. ISO increased total I_Ca, I_Ca-T and I_Ca-L at most membrane potentials. ISO shifted the voltage at which I_Ca-L peaked from 0 mV to −10 mV but did not change the voltage (−40 mV) at which I_Ca-T(3.1) peaked. <b>F</b> and <b>G</b>: the voltage-dependent activation and inactivation curves of I_Ca-L and I_Ca-T before and after the application of ISO. @: <i>p<0.001;</i> #: <i>p<0.01; $: p<0.05;</i> TG vs. control at the same test potential. Statistics were done with two-way ANOVA and post-hoc t-test.