<p>Seven ascomycete and seven basidioimycete species were subjected to amplification of the (a) ITS1 region (ITS1-F_KYO–ITS2_KYO2), (b) ITS2 region (ITS3_KYO2–ITS4), and the (c) entire ITS region (ITS1-F_KYO2–ITS4) at 47°C (electrophoresed on 2.2% agarose gels). See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0040863#pone-0040863-t002" target="_blank">Table 2</a> for the abbreviations of fungal specimens. N, negative control.</p
<p>Primers UNI1, UNI2, and Cspecies, and probe are used in the qPCR strategy. Primers Fseq and Rseq ...
The study of fungal diversity may lead to many fundamental discoveries and conclusions. Molecular ge...
454 pyrosequencing; community structure; species richness; Sørensen index; minimum read length. The ...
<p>Three ascomycete and four basidiomycete species were subjected to amplification of the (a) ITS1 r...
The kingdom Fungi is estimated to include 1.5 million or more species, playing key roles as decompos...
The kingdom Fungi is estimated to include 1.5 million or more species, playing key roles as decompos...
FIG. 6. — Results of PCR amplifications using discriminant probes for Amanita pseudovalens comb. nov...
This paper presents an in silico analysis to assess the current state of the fungal UNITE database i...
<p>The percentage of fungal LTUs amplified by ecoPCR is shown for each primer. In each analysis, <i>...
<p>Sequences of primers developed in this study to amplify specifically the ITS region of fungal Mol...
APPENDIX 2. — Example of a matched comparison of Amanita ponderosa Malençon & R.Heim (ApoIf2-ITS4) a...
Current metabarcoding studies aiming to characterize microbial communities generally rely on the amp...
The impact of primer choice on results of metabarcoding studies was experimentally evaluated by anal...
Metabarcoding has become an important tool in the discovery of biodiversity, including fungi, which ...
Figure 7 - Differences in sequence length in the ITS1 and ITS2 barcodes of 16 most abundant fungal c...
<p>Primers UNI1, UNI2, and Cspecies, and probe are used in the qPCR strategy. Primers Fseq and Rseq ...
The study of fungal diversity may lead to many fundamental discoveries and conclusions. Molecular ge...
454 pyrosequencing; community structure; species richness; Sørensen index; minimum read length. The ...
<p>Three ascomycete and four basidiomycete species were subjected to amplification of the (a) ITS1 r...
The kingdom Fungi is estimated to include 1.5 million or more species, playing key roles as decompos...
The kingdom Fungi is estimated to include 1.5 million or more species, playing key roles as decompos...
FIG. 6. — Results of PCR amplifications using discriminant probes for Amanita pseudovalens comb. nov...
This paper presents an in silico analysis to assess the current state of the fungal UNITE database i...
<p>The percentage of fungal LTUs amplified by ecoPCR is shown for each primer. In each analysis, <i>...
<p>Sequences of primers developed in this study to amplify specifically the ITS region of fungal Mol...
APPENDIX 2. — Example of a matched comparison of Amanita ponderosa Malençon & R.Heim (ApoIf2-ITS4) a...
Current metabarcoding studies aiming to characterize microbial communities generally rely on the amp...
The impact of primer choice on results of metabarcoding studies was experimentally evaluated by anal...
Metabarcoding has become an important tool in the discovery of biodiversity, including fungi, which ...
Figure 7 - Differences in sequence length in the ITS1 and ITS2 barcodes of 16 most abundant fungal c...
<p>Primers UNI1, UNI2, and Cspecies, and probe are used in the qPCR strategy. Primers Fseq and Rseq ...
The study of fungal diversity may lead to many fundamental discoveries and conclusions. Molecular ge...
454 pyrosequencing; community structure; species richness; Sørensen index; minimum read length. The ...