FIG. 6 in The Amidella clade in Europe (Basidiomycota: Amanitaceae): clarification of the contentious Amanita valens (E.-J.Gilbert) Bertault and the importance of taxon-specific PCR primers for identification

FIG. 6. — Results of PCR amplifications using discriminant probes for Amanita pseudovalens comb. nov., stat. nov.: A, ITS region: ITS3-ApsIr3 (all samples positive with concurring control with primers ITS3-ITS4, not shown); B, LSU region: NLC2R-ApsLr2 (all positive with control NLC2R-LR5); Ode15 and Ode16 were negative and positive, respectively, in another amplification (not shown); M, molecular marker; neg. designates the negative control (water); pos. designates the positive control (the P01 extract).Published as part of Arraiano-Castilho, Ricardo, Silva, Ana Cristina, Vila-Viçosa, Carlos, Castro, Mário Rui, Morgado, Luís Neves & Oliveira, Paulo, 2022, The Amidella clade in Europe (Basidiomycota: Amanitaceae): clarification of the contentious Amanita valens (E.-J.Gilbert) Bertault and the importance of taxon-specific PCR primers for identification, pp. 139-157 in Cryptogamie, Mycologie 20 (6) on page 151, DOI: 10.5252/cryptogamie-mycologie2022v43a6, http://zenodo.org/record/782951