Effect of enzyme inhibitors on cell proliferation and migration of MDA-MB-231 cells.

<p>MDA-MB-231 tumor cells treated with either vehicle or roziglitazone (Ros), troglitazone (Trog), Triasin C (TriC), zileuton (Zil), AA861, ibuprofen (Ibu), etoricoxib (Eto) as ACSL4, LOX-5 and COX-2 inhibitors, respectively, alone or in combination as indicated were incubated during 12 or 96 h and cell migration and proliferation assayed as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0040794#s4" target="_blank">Materials and Methods</a>.Doses employed were: 25 µM for Ros and Trog; 100 µM and 5 µM for Zil and AA861 respectively; 100 µM and 0.01 µM for Ibu and Eto respectively and 5 µM for TriC.</p><p>Data represent the mean ± SD of 2 or 3 independent experiments for cell migration and proliferation, respectively, performed in quintuplicate. * P<0.05 and ** P<0.01 vs. Control.