Cell migration.

<p>Inhibition of tumour migration by a Boyden chamber assay. CaCo-2, HT29 and SW480 cells were plated onto the apical side of Matrigel-coated filters in serum-free medium supplemented with drugs at different concentrations (0.1, 1, 10, 50 µM rosiglitazone; 0.1, 1, 10, 50 µM AS601245) and with the association of the drugs at different concentrations for 24 hours. Chemoattractant utilized was 20% FCS supplemented medium, placed in the basolateral chamber. The cells migrated to the bottom of the filters were stained using crystalviolet and counted (5 fields of each triplicate filters) using an inverted microscope. Control migration was 45±8 cells/microscope fields for CaCo-2 cells, 50±5 cells/microscope fields for HT29 and 40±3 cells/microscope fields for SW480. Data are expressed as mean±SEM (n = 5) of the percentage of inhibition versus the migration of cells exposed to vehicle (1% DMSO). Variance analysis *<i>p</i><0.05, **<i>p</i><0.01 vs control; a <i>p</i><0.05, aa <i>p</i><0.01 vs rosiglitazone; b <i>p</i><0.05, bb <i>p</i><0.01 vs AS601245.