Interaction between endogenous Nedd4 and MAK-V proteins.

<p>Proteins were immunoprecipitated from lysates of CSML-0 cells with anti-Nedd4 (<b>A</b>; <i>IP: Nedd4 Ab</i>) or anti-MAK-V antibodies (<b>B</b>; <i>IP: MAK-V Ab</i>). Control immunoprecipitations with omitted antibodies were run in parallel (<i>IP: control</i>). Immunoprecipitates and aliquots of lysates used for immunoprecipitation (<i>Input</i>) were probed with anti-MAK-V (<i>anti-MAK-V</i>) and anti-Nedd4 (<i>anti-Nedd4</i>) antibodies to detect MAK-V and Nedd4 proteins, respectively.