<p><i>A</i>. HEK293 cells were transfected with the indicated constructs. At 48 h after post-transfection, cells were treated for 6 h with DMSO (-) or Roscovitine (+; 20 µM) and then lysed, and the FLAG-tagged proteins were immunoprecipitated with anti-FLAG beads. The precipitates were analyzed by immunoblotting with the indicated antibodies. <i>B.</i> HEK293 cells were transfected with the indicated constructs. The FLAG-tagged proteins were immunoprecipitated from the cell lysates with anti-FLAG beads. The precipitates were analyzed by immunoblotting with the indicated antibodies.</p
<p>(<b>A</b>) E2 enzyme specificity of ubiquitin-ligase activity co-purified with MAK-V-FLAG protein...
<p>(A) Full length Myc-NEDD1 transfected HEK293T cells were incubated with and without DSS crosslink...
<p>(<b>A</b>) Hexa-histidine tagged wild-type Cif (WT), Cif cysteine 109 mutant Cys-to-Ser (C/S), Ci...
<p><i>A</i>. HEK293 cells were transfected with the indicated constructs. FLAG-tagged proteins were ...
<p><i>A.</i> Schematic diagram of the amino acids in Kif26b potentially phosphorylated by CDK5. The ...
<p>(<b>A</b>) Typical profile of proteins purified on anti-FLAG M2 affinity gel from PC12TetOn cells...
<p>(<b>A</b>) HEK293 cells were transfected with MAK-V-FLAG expression plasmid (<i>MAK-V-FLAG +</i>)...
<p>A-C) HEK293T cells were co-transfected with plasmids expressing myc-hIFITM3 or FLAG-NEDD4 as indi...
<p>Endogenous Daxx in U2OS cells was detected following Mock transfection (A), transfection with VI-...
<p>(A) ΔN60SGK-HA and Nedd4-2<sub>C821A</sub>–FLAG were transiently co-expressed, or expressed alone...
<p>293 cells were transfected with HXB2 and a plasmid encoding either rat Nedd4 (rNedd4) or a rat Ne...
<p>Proteins were immunoprecipitated from lysates of CSML-0 cells with anti-Nedd4 (<b>A</b>; <i>IP: N...
<p>(<b>A</b>) Akt associates with and phosphorylates Klf4. Reciprocal immunoprecipitations were carr...
The HECT E3 ubiquitin ligase Nedd4-1 was previously shown to regulate diverse processes such as cell...
<p>(A) The interaction of endogenous NEDD1 and γ-tubulin was assessed in HEK293T cells. Due to its l...
<p>(<b>A</b>) E2 enzyme specificity of ubiquitin-ligase activity co-purified with MAK-V-FLAG protein...
<p>(A) Full length Myc-NEDD1 transfected HEK293T cells were incubated with and without DSS crosslink...
<p>(<b>A</b>) Hexa-histidine tagged wild-type Cif (WT), Cif cysteine 109 mutant Cys-to-Ser (C/S), Ci...
<p><i>A</i>. HEK293 cells were transfected with the indicated constructs. FLAG-tagged proteins were ...
<p><i>A.</i> Schematic diagram of the amino acids in Kif26b potentially phosphorylated by CDK5. The ...
<p>(<b>A</b>) Typical profile of proteins purified on anti-FLAG M2 affinity gel from PC12TetOn cells...
<p>(<b>A</b>) HEK293 cells were transfected with MAK-V-FLAG expression plasmid (<i>MAK-V-FLAG +</i>)...
<p>A-C) HEK293T cells were co-transfected with plasmids expressing myc-hIFITM3 or FLAG-NEDD4 as indi...
<p>Endogenous Daxx in U2OS cells was detected following Mock transfection (A), transfection with VI-...
<p>(A) ΔN60SGK-HA and Nedd4-2<sub>C821A</sub>–FLAG were transiently co-expressed, or expressed alone...
<p>293 cells were transfected with HXB2 and a plasmid encoding either rat Nedd4 (rNedd4) or a rat Ne...
<p>Proteins were immunoprecipitated from lysates of CSML-0 cells with anti-Nedd4 (<b>A</b>; <i>IP: N...
<p>(<b>A</b>) Akt associates with and phosphorylates Klf4. Reciprocal immunoprecipitations were carr...
The HECT E3 ubiquitin ligase Nedd4-1 was previously shown to regulate diverse processes such as cell...
<p>(A) The interaction of endogenous NEDD1 and γ-tubulin was assessed in HEK293T cells. Due to its l...
<p>(<b>A</b>) E2 enzyme specificity of ubiquitin-ligase activity co-purified with MAK-V-FLAG protein...
<p>(A) Full length Myc-NEDD1 transfected HEK293T cells were incubated with and without DSS crosslink...
<p>(<b>A</b>) Hexa-histidine tagged wild-type Cif (WT), Cif cysteine 109 mutant Cys-to-Ser (C/S), Ci...