Identification of Nedd4 as a Kif26b-interacting protein.

<p><i>A</i>. HEK293 cells were transfected with the indicated constructs. FLAG-tagged proteins were immunoprecipitated from the lysates with anti-FLAG beads. The precipitates were analyzed by immunoblotting with the indicated antibodies. <i>B</i>. Schematic diagrams of the Kif26b deletion constructs. Interaction with Nedd4 is summarized on the <i>right</i> of each construct: +, positive; −, negative. <i>C</i>. HEK293 cells were transfected with the indicated constructs. FLAG-tagged proteins were immunoprecipitated from the lysates with anti-FLAG beads. The precipitates were analyzed by immunoblotting with the indicated antibodies. <i>D</i>. Schematic diagrams of the Nedd4 deletion constructs. Interaction with Kif26b is summarized on the right of each construct: +, positive; −, negative. <i>E</i>. HEK293 cells were transfected with FLAG-Kif26b-expressing plasmids. The lysates were incubated with purified GST or GST-tagged Nedd4 deletions. The glutathione bead precipitates were analyzed by immunoblotting with the indicated antibodies.