Effect of Linker replacement in Rep68 oligomerization.
- Francisco Zarate-Perez (319391)
- Martino Bardelli (319392)
- John W. Burgner II (319393)
- Maria Villamil-Jarauta (319394)
- Kanni Das (319395)
- Demet Kekilli (319396)
- Jorge Mansilla-Soto (262628)
- R. Michael Linden (18009)
- Carlos R. Escalante (262635)
Publication date
February 2013
DOIAbstract
<p>Comparison of sedimentation profiles of Rep68 and Rep68<sub>octlink</sub> proteins. (A) Rep68 sediments as a major peak at ∼13S. (B) Rep68<sub>octlink</sub> sediments as a monomer with sedimentation coefficient of 3.5S. (C) Rep68<sub>octlink</sub> in presence of 5 mM ATP sediments in two peaks corresponding to monomer and dimer species. Protein concentration was kept constant at 25 µM in buffer containing 200 mM NaCl. Sedimentation velocity experiments were run at 40000 rpm and 20°C. Data was collected using the interference system.</p
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