<p>(A) 12.5% SDS-PAGE. (B) 10% native-PAGE. (C) Activity staining of the bands in native-PAGE. M is the protein marker, TT is tetramer and O is high-order oligomers. The proteins were dissolved in 20 mM PBS buffer, pH 7.4.</p
Over half the proteins in the E. coli cytoplasm form homo or hetero-oligomeric structures. Experimen...
<p>The oligomerization state of Rco<sub>LS20</sub> protein in solution was studied by two complement...
The chapter is a wide overview focused on protein oligomerization, a topic that definitely increase...
<p>(A) Salt-dependence. (B) pH-dependence. (C) Heat treatment at 80°C. (D) Heat treatment at 95°C. T...
<p>Most of the high-order oligomers were quickly dissociated into tetramers after collection. The pu...
<p>(A) Native-PAGE. (B) Activity staining. (C) Stability at 95°C. The protein concentration was 1 mg...
<p>Standard curve <i>Ve/Vo versus</i> log molecular size markers is shown in the left most panel. <i...
<p>Size exclusion elution profiles from a Superdex 200 column are displayed. <b>A</b>, Concentrated ...
<p>Analytical SEC and nESI mass spectra of the different protein constructs measured at the same pro...
<p>(A) Size exclusion chromatography was performed with the PHB domain on a Superose6 column (GE Hea...
<p>(A) Bis-tris−HEPES−MES buffer systems; (B) HEPES−Imidazole/Bis-tris buffer systems; (C) Imidazole...
A. SEC chromatograms of MBPCC1 at 4˚, 24˚, and 32˚C. The instrument and column for T = 24˚C and T = ...
<p>Elution chromatograms of purified HpDprA at 2 mg/ml and 1 mg/ml <b>(A)</b> concentrations are dep...
<p><b>A</b>. Recombinant fluorescent proteins electrophoresed on a SDS-containing gel without prior ...
<p>(A) Bis-tris−HEPES−MES buffer systems; (B) HEPES−Imidazole/Bis-tris buffer systems; (C) Imidazole...
Over half the proteins in the E. coli cytoplasm form homo or hetero-oligomeric structures. Experimen...
<p>The oligomerization state of Rco<sub>LS20</sub> protein in solution was studied by two complement...
The chapter is a wide overview focused on protein oligomerization, a topic that definitely increase...
<p>(A) Salt-dependence. (B) pH-dependence. (C) Heat treatment at 80°C. (D) Heat treatment at 95°C. T...
<p>Most of the high-order oligomers were quickly dissociated into tetramers after collection. The pu...
<p>(A) Native-PAGE. (B) Activity staining. (C) Stability at 95°C. The protein concentration was 1 mg...
<p>Standard curve <i>Ve/Vo versus</i> log molecular size markers is shown in the left most panel. <i...
<p>Size exclusion elution profiles from a Superdex 200 column are displayed. <b>A</b>, Concentrated ...
<p>Analytical SEC and nESI mass spectra of the different protein constructs measured at the same pro...
<p>(A) Size exclusion chromatography was performed with the PHB domain on a Superose6 column (GE Hea...
<p>(A) Bis-tris−HEPES−MES buffer systems; (B) HEPES−Imidazole/Bis-tris buffer systems; (C) Imidazole...
A. SEC chromatograms of MBPCC1 at 4˚, 24˚, and 32˚C. The instrument and column for T = 24˚C and T = ...
<p>Elution chromatograms of purified HpDprA at 2 mg/ml and 1 mg/ml <b>(A)</b> concentrations are dep...
<p><b>A</b>. Recombinant fluorescent proteins electrophoresed on a SDS-containing gel without prior ...
<p>(A) Bis-tris−HEPES−MES buffer systems; (B) HEPES−Imidazole/Bis-tris buffer systems; (C) Imidazole...
Over half the proteins in the E. coli cytoplasm form homo or hetero-oligomeric structures. Experimen...
<p>The oligomerization state of Rco<sub>LS20</sub> protein in solution was studied by two complement...
The chapter is a wide overview focused on protein oligomerization, a topic that definitely increase...
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