The resolution of high and low molecular mass protein markers (SDS) by the PAGE in the three buffer systems.

<p>(A) Bis-tris−HEPES−MES buffer systems; (B) HEPES−Imidazole/Bis-tris buffer systems; (C) Imidazole−Acetic acid buffer systems. The PAGE was performed with a gradient resolving gel of 5%–20% in pH 7.0 and a stacking gel of 3% in pH 6.0, and 0.1% SDS was added in cathode buffers. The band patterns of each PAGE were visualized by Coomassie Blue G-250.