Sedimentation velocity experiments of GFP-tagged EB1 proteins.

<p>Sedimentation coefficient distribution profiles of EB1-GFP (A) and EB1c-GFP (B) at the different protein concentrations indicated in the corresponding legends. The I224A mutant versions of the proteins are also shown as a reference. Sedimentation coefficient positions of monomers (M) and dimers (D) are indicated. (<b>C</b>) and (<b>D</b>) Sedimentation coefficients (symbols) plotted against protein concentration for EB1-GFP and EB1[I224A]-GFP (C), and EB1c-GFP and EB1c[I224A]-GFP (D). The lines represent the linear fits to the data.