Sedimentation velocity and SAXS analysis of GRP78 and PAT-SM6.^a

a<p>Sedimentation coefficients (S_{20, w}) and frictional ratios (f/f₀) were calculated from fitting experimental sedimentation velocity data to a continuous size distribution c(s) model. These values were used to calculate the molecular weight (MW) and to model the protein shape as an oblate ellipsoid with axial dimensions a (nm) and b (nm). SAXS data for GRP78 and PAT-SM6 (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0044927#pone-0044927-g002" target="_blank">Figures 2</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0044927#pone-0044927-g004" target="_blank">4</a>, respectively) was analysed by Guinier analysis to estimate radius of gyration, <i>R_g</i> and <i>P</i>(<i>r</i>) analysis to estimate maximum dimension, <i>D_max</i>.