Comparison of gene transcript levels in mouse livers following HNF4α knockdown and liver-specific targeted deletion of <i>Hnf4a</i>.

<p>Livers from siHNF4α injected animals (panel A, black bars) and siNEG injected animals as controls (panel A, open bars) were subjected to control and coagulation gene transcript levels by QPCR. In panel B, coagulation transcript analysis for liver materials from HNF4α-null mice (KO) or control mice (FLOX) is included for comparison <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038104#pone.0038104-Hayhurst1" target="_blank">[25]</a>. Data are presented for mice two days post siRNA injection or app. 6 weeks gene ablation (i.e. 6 weeks old HNF4α-null mice). β-actin was used as internal control for quantification and normalization. The ΔCt values of the individual samples were related to the mean ΔCt of the siNEG or FLOX control group. Data are expressed as mean with error bars representing the difference between 2 POWER of upper and lower range of the mean ΔΔCt (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038104#pone-0038104-g001" target="_blank">figure 1</a> legends). Data were statistically analysed using Mann Whitney Rank Sum test. <i>P</i>-values<0.05 were regarded as statistically significant. *<i>P</i><0.05, **<i>P</i><0.01, ***<i>P</i><0.001. On the x-axis the coagulation genes are ranked according to the magnitude of effects observed in the siRNA injected animals.