Hepatic coagulation gene transcription following HNF4α and C/EBPα knockdown.

<p>At two (upper panels) and five days (lower panels) post siRNA injection, mouse livers were subjected to coagulation gene transcript levels by QPCR. Data are presented for siHNF4α (panel A and C, black bars) and siCEBPα (panel B and D, hatched bars) with siNEG injected animals as controls (panel A, B, C and D, open bars). β-actin was used as internal control for quantification and normalization. The ΔCt values of the individual samples were related to the mean ΔCt of the siNEG group. Data are expressed as mean with error bars representing the difference between 2 POWER of upper and lower range of the mean ΔΔCt (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038104#pone-0038104-g001" target="_blank">figure 1</a> legends). Data were statistically analysed using Mann Whitney Rank Sum test. <i>P</i>-values<0.05 were regarded as statistically significant. *<i>P</i><0.05, **<i>P</i><0.01, ***<i>P</i><0.001. Only those coagulation genes are presented for which transcript levels were significantly affected by siHNF4α or siC/EBPα (in two or five days study group). On the x-axis the coagulation genes are ranked according to the magnitude of effects observed in the two day groups.