There are two <i>X. laevis</i> TIMP2 genes that are highly homologous to the TIMP2 gene in other vertebrates.

<p>A) Nucleotide sequence comparison of <i>X. laevis</i> TIMP2A and TIMP2B. Dot (.): identical sequence; dash (-):a gap introduced for better alignment. Arrowed lines indicate the locations of the primers used to distinguish the two isoforms in RT-PCR analysis. B) Comparison of the protein sequences of putative <i>X. laevis</i> TIMP2A (XlTIMP2A, AAH74452), <i>X. laevis</i> TIMP2B (XlTIMP2B, NP_001087748), and <i>X. tropicalis</i> TIMP2 (XtTIMP2, NP_001015760) with their ortholog from chick (ChTIMP2, NP_989629), mouse (mTIMP2, P25785) and human (HuTIMP2, NP_003246). The conserved cysteine sequences are in bold and the signal peptide sequence is underlined. Dot (.): identical sequence; dash (-): a gap introduced for better alignment.