Amino acid sequence alignment of chicken CART1/2 with CART3-6 from other species.

<p>Amino acid sequence alignment of chicken CART1 (KC249966) and CART2 (KC249967) precursors with that of CART3 precursor from painted turtles (XM_005300628), <i>Xenopus tropicalis</i> (NM_001079103), tilapia (CART3a: XM_003456893; CART3b: XM_003449187) and coelacanth (XM_005989732), or CART4 precursor from <i>Xenopus tropicalis</i> (XM_002932246), tilapia (XM_005461629), coelacanth, and elephant shark (XM_007908392), or CART5 precursor from takifugu (XM_003969141), tilapia (XM_003455220), tetraodon, and coelacanth, or CART6 precursor from green sea turtles (EMP28211) and coelacanth. The conserved dibasic residues (KR/KK) for proteolytic processing are marked by two arrows presented in proCARTs. Lines linking cysteines indicate the three conserved disulfide bonds. CART sequences from species other than the ones cloned in chickens were either predicted according to their genomic sequences, or retrieved from GenBank. Asterisks and dots indicate the signature motifs (KCI or RCY) possessed by vertebrate CART4 and CART5 respectively.