Sequence comparison of TRPM8 species orthologs.

<p>(A) Previously described rat, human, and chicken TRPM8 sequences were aligned with the full-length sequences of <i>X. tropicalis</i> and <i>X. laevis</i> TRPM8 (this study) using MultAlin and ESPript. The locations of predicted transmembrane helices <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0005741#pone.0005741-McKemy1" target="_blank">[6]</a> and the C-terminal coiled-coiled assembly domain <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0005741#pone.0005741-Tsuruda1" target="_blank">[25]</a> are shown as black and gray bars, respectively. The asterisk indicates the polymorphic residue previously shown to determine TRPM8 icilin sensitivity <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0005741#pone.0005741-Chuang1" target="_blank">[17]</a>. (B) Phylogenetic tree indicating the evolutionary relationship between TRPM8 ortholog sequences.