EYA2 overexpression in MCF10A cells increases cell motility, which in turn is inhibited by EYA inhibitors.

<p>(<b>a</b>) Trans-well migration of MCF10A cells transfected with either pcDNA 3.2/V5-DEST (V5 vector control), V5-EYA2, or the phosphatase dead mutant V5-EYA2(mut). (<b>b</b>) MCF10A-EYA2 cells were treated with 7.5 μM of compounds 1, 1a, 1b, or 1c. Percentage migration relative to cells treated with the vehicle (0.1% DMSO) is shown. (<b>c</b>) Transwell migration of MCF10A-EYA2(mut) cells in the presence of 7.5 μM of compounds 1, 1a, 1b, or 1c (<b>d</b>) Relative migration of MCF10A-EYA2 cell migration by compounds 1, 1a, and 1b when treated with the doses indicated on the x-axis. (<b>e</b>) Change in cell density of MCF10A-V5 ctl, MCF10A-EYA2 and MCF10A-EYA2(mut) cells after 48 hours measured using the MTT assay. (<b>f</b>) Change in cell density of MCF10A-EYA2 cells after 48 hours in the presence of either vehicle control (0.1% DMSO) or 10 μM compounds 1, 1a, and 1b. For transwell migration experiments each bar represents the mean (and standard error) of five random fields per filter and two wells per experiment. For proliferation experiments each bar represents the mean (and standard error) of three experiments. The <i>p</i>-values were calculated by one-way ANOVA followed by a Tukey post test. ns is not significant, * <i>p</i><0.05, ** <i>p</i><0.01, *** <i>p</i><0.001. In each case the <i>p</i> value shown is relative to the V5-control or the vehicle-treated sample.