Effect of the Arp 2/3 inhibitor CK-869 and Arp3 knockdown on migration of MCF 10A cells.

<p>(<b>A</b>) Traces of 6 individual trajectories of MCF10A cells plotted after aligning their starting positions for wildtype cells and cells in 12.5, 25 and 50 µM of CK-869 or siRNA Arp3 cells. Scale bar is 100 µm. (<b>B</b>) Fraction of cells that are motile (move greater than 1 cell diameter over 10 hours) or non-motile (move less than 1 cell diameter over 10 hours). Motile cells are segregated into those that have a d/t (d is overall distance traveled; t is total path length) greater or less than 0.5 to identify persistent and non-persistent migration, respectively. (n = 22, 50, 50, 50, 50, 50 and 29 cells respectively).(<b>C</b>) Instantaneous migration speed for motile cells in same conditions described in A. (N = 19, 38, 45, 31, 36, 50 and 19 cells respectively). Error bars = SD. NS, not significant; **, P<0.01; ***, P<0.001 with respect to WT or control.