Pattern of NADPH-d labeling in Purkinje cells at P9 shown here after gray-scale conversion of the original color image.

<p>The scheme in the uppermost part of the figure indicates in bold and italic the folia shown in the figures A to H, at the vermis region (0.5 mm mediolateral distance). Med - medial (fastigial) cerebellar nucleus; Pcuf - preculminate fissure; Prf - primary fissure; Psf - posterior superior fissure; Ppf - prepyramidal fissure; Sf - secondary fissure; Uf - uvular fissure Plf - posterolateral fissure; ML - molecular layer. A, B: At folium 3 in SHAM animals (A), Purkinje cell bodies have a strong labeling and the dendritic trees are well defined by NADPH-d staining (arrows), while in HI animals (B), cell bodies have a weaker staining with a less defined staining pattern in the dendritic arbors (arrows). C, D: At folium 1 in SHAM animals (C), both cell bodies and processes have a strong labeling (arrowheads), while in HI ones (D), the cell bodies do not present NADPH-d activity (asterisks in the presumptive PC). Observe in D that the ML presents a strong and diffuse staining that corresponds to the location of the dendritic trees of the Purkinje cells. E, F: At folium 10, none of the groups presented NADPH-d staining in Purkinje cell bodies or in the dendritic arbors (asterisks in the presumptive PC). The presence of Purkinje cells was verified by hemaetoxilin staining in an adjacent section (G – SHAM; H – HI, arrows). WM = white matter; IGL = internal granular layer; EGL = external granular layer. Calibration bar: A–D, G and H = 50 µm, E and F - 100 µm.