Golgi Stains Illustrating Dendritic Abnormalities in <i>Bcl-w^−/−Grid2^{ho−4J(−/−)}</i> Double Mutant Mice.

<p>(A) Golgi stain showing the profile of Purkinje cell dendritic trees in wild type, <i>Bcl-w^−/−</i>, <i>Grid2^{ho−4J(−/−)},</i> and <i>Bcl-w^−/−Grid2^{ho−4J(−/−)}</i> mice (top panel). Low magnification view of one partial folia in the <i>Bcl-w^−/−Grid2^{ho−4J(−/−)}</i> cerebellum illustrating several affected Purkinje cells (lower panel). Red arrows indicate two primary dendritic branches extended from the Purkinje cell soma. (B) The Strahler order of method. This method was designed to assess topological features of dendrites by assigning a relative order of magnitude to each dendritic branch. The number of Strahler orders, combined with the number of branches in each of these orders, is a quantitative measure of the complexity of a dendritic tree. The tip branches are counted as “order 1”, and two “order 1” branches meet to form the “order 2” branch, etc.