Immunofluorescence of cerebellar sections at increasing magnifications illustrates the relationship between calbindin-positive (green) Purkinje cell bodies, their dendrites and VGLUT1-positive (red) parallel fibres and synaptic terminals in the molecular layer, NBH-animals at 18 weeks p.i.

<p>The calbindin staining (green) reveals the Purkinje cells and the arborisation of their major processes in the molecular layer. The blue panel is DAPI staining of the cell bodies and the fourth panel shows the overlay of the three colours. VGLUT1 immunoreactivity (red) was abundantly present throughout the molecular layer and also in the granular layer associated with the cell somas of the granule cells (nuclear stain blue) that give rise to the parallel fibres. Scale bars: 400 µm (A); 112 µm (B); 57 µm (C).