Analysis of T₀<i>N. tabacum</i> LAFC 53 plant lines transformed with HA constructs.

<p>2a. The mean HA content in <i>Nt</i> LAFC-HA plants transgenic for each construct type quantified using ELISA analysis. *Mean levels of HA in plants transgenic for pDAB4493 were significantly different (p&lt;0.05; t-test) to those containing pDAB4492. Nt = non-transgenic <i>N. tabacum</i> var. LAFC 53. Error bars represent standard error of mean (SEM) (n = 40 for plants transgenic for pCHA; n = 45 for plants transgenic for pDAB4492; n = 52 for plants transgenic for pDAB4493), FW = fresh weight. 2b. Western analysis of leaf extracts from representative <i>Nt</i> LAFC-HA plants. Extracts from plants containing HA constructs were loaded in central lanes as indicated. Purified HA was loaded as a control in the left lane whilst leaf extract from non-transgenic <i>N. tabacum</i> var. LAFC 53 was loaded in the right lane. All lanes contained 10 µg total soluble protein (TSP). 2c. Detection of HA transgene in selected elite transgenic lines <i>of N. tabacum</i> containing the pDAB4493-HA construct. Southern blot hybridisation of <i>Hind</i> III digested genomic DNA isolated from Nt LAFC-HA transgenic plants probed with the HA gene.