<p>(A) A schematic diagram of the mAb SO57 HC and LC DNA constructs <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0068772#pone.0068772-Brodzik1" target="_blank">[7]</a>. (B) PCR analysis of LC (729 bp), HC (1431 bp) and HC fused to KDEL (HCK, 1443 bp) in the genomic DNA. NT, non-transgenic plant; mAb<sup>P</sup>, non-KDEL-tagged mAb<sup>P</sup>; mAb<sup>P</sup> K, KDEL- tagged mAb<sup>P</sup>.</p
<p>2a. The mean HA content in <i>Nt</i> LAFC-HA plants transgenic for each construct type quantified...
<p>(a) Schematic of T-DNA for vectors pCAMBIA-<i>PAP1</i> (upper), <i>Lc</i> (middle) and <i>PAP1</i...
<p>Genomic DNA isolated from selected transgenic lines derived from the two constructs and untransfo...
<p>Purified mAbs were loaded onto the gels and stained with Coomassie brilliant blue R250. TPS, tota...
<p>(A) Relative expression levels (Y axis) of the HC and LC gene as determined by RT-qPCR. Amplicons...
(A) RT-PCR and (B) western blot analyses were performed to confirm HC and LC expression based on RNA...
<p><b>A</b>) PCR detection of the gene <i>man5As</i>. Lane 1, the DNA molecular weight markers; lane...
<p><b>A</b> PCR detection of the gene <i>bgl7Am</i> in the genomic DNA of transgenic plant leaves of...
Recombinant therapeutic monoclonal antibodies (mAb) can be expressed, assembled, and glycosylated in...
<p>(a) Schematic map of plant expression gene construct pCAMBIA1301-<i>SbMT-2</i>. (b) Confirmation ...
<p>(A) Schematic representation of A56 genetic cassette used for tobacco plant transformation. The <...
<p>(A) and (B) PCR analysis for the presence of the <i>Cry1Ac</i> and <i>SCK</i> gene in transgenic ...
<p>(<b>A</b>) Schematic diagram of the plant expression vectors pAMM2024 and pAMM2025, which are der...
<p>(a) Schematic diagram of the <i>35S::JcLEA</i> DNA vector construct. (b) Verification of transfor...
<p>(a) DNA gel blot analysis of three transgenic plants containing a single copy of the target locus...
<p>2a. The mean HA content in <i>Nt</i> LAFC-HA plants transgenic for each construct type quantified...
<p>(a) Schematic of T-DNA for vectors pCAMBIA-<i>PAP1</i> (upper), <i>Lc</i> (middle) and <i>PAP1</i...
<p>Genomic DNA isolated from selected transgenic lines derived from the two constructs and untransfo...
<p>Purified mAbs were loaded onto the gels and stained with Coomassie brilliant blue R250. TPS, tota...
<p>(A) Relative expression levels (Y axis) of the HC and LC gene as determined by RT-qPCR. Amplicons...
(A) RT-PCR and (B) western blot analyses were performed to confirm HC and LC expression based on RNA...
<p><b>A</b>) PCR detection of the gene <i>man5As</i>. Lane 1, the DNA molecular weight markers; lane...
<p><b>A</b> PCR detection of the gene <i>bgl7Am</i> in the genomic DNA of transgenic plant leaves of...
Recombinant therapeutic monoclonal antibodies (mAb) can be expressed, assembled, and glycosylated in...
<p>(a) Schematic map of plant expression gene construct pCAMBIA1301-<i>SbMT-2</i>. (b) Confirmation ...
<p>(A) Schematic representation of A56 genetic cassette used for tobacco plant transformation. The <...
<p>(A) and (B) PCR analysis for the presence of the <i>Cry1Ac</i> and <i>SCK</i> gene in transgenic ...
<p>(<b>A</b>) Schematic diagram of the plant expression vectors pAMM2024 and pAMM2025, which are der...
<p>(a) Schematic diagram of the <i>35S::JcLEA</i> DNA vector construct. (b) Verification of transfor...
<p>(a) DNA gel blot analysis of three transgenic plants containing a single copy of the target locus...
<p>2a. The mean HA content in <i>Nt</i> LAFC-HA plants transgenic for each construct type quantified...
<p>(a) Schematic of T-DNA for vectors pCAMBIA-<i>PAP1</i> (upper), <i>Lc</i> (middle) and <i>PAP1</i...
<p>Genomic DNA isolated from selected transgenic lines derived from the two constructs and untransfo...