Analyses of transgenic tobacco plants.

<p>(a) Schematic map of plant expression gene construct pCAMBIA1301-<i>SbMT-2</i>. (b) Confirmation of transgenic lines by PCR amplification of <i>uidA</i>, <i>hptII</i> and <i>SbMT-2</i> genes (Lane M is molecular weight markers, Lane PC is positive control of PCR using plasmid <i>pCAMBIA1301-SbMT-2</i> as template, Lane WT is wild type control plant (non-transgenic) and Lane L is transgenic lines). (c) Over-expression of the <i>SbMT-2</i> gene in transgenic lines compared to wild-type control plants (Lane WT is wild type control plant and Lane L is transgenic lines) analyzed by semi-quantitative Rt-PCR, where <i>actin</i> was used as internal gene control. (d) Histochemical GUS staining of WT and transgenic plants. (e) Southern blot analysis (Lane PC is positive control plasmid <i>pCAMBIA1301-SbMT-2</i>, Lane WT is wild type control plant (non-transgenic) and Lane L is transgenic lines).