Confirmation of transgenic tobacco plants.

<p>(A) Schematic representation of the pCAMBIA2301-35S:SbSI-2 construct used to transform tobacco plants with the <i>SbSI-2</i> gene, (B) GUS assay of seedlings, showing positive GUS expression in the transgenic lines, (C) Transcript levels of the <i>SbSI-2</i> gene in transgenic lines and WT plants via semi-quantitative RT-PCR, (D) Southern analysis of transgenic lines, (E–F) Germination of seeds from transgenic lines (L11, L17 and L22) and WT plants in (E) 0 mM, and (F) 200 mM NaCl and (G) Graphs represent the percentage germination of transgenic lines (L11, L17 and L22) and WT plants in salt stress and normal condition. Mean values that were significantly different at p≤0.05 within treatment from each other are indicated by different letters (a, b and c).